Abstract
Introduction and Aim: India has the uppermost trouble of Multidrug resistant tuberculosis (MDR-TB) is a major challenge controlling resistance, reducing transmission and improving handling outcomes in MDR-TB patients is dependent on susceptibility testing. Isoniazid (INH) and rifampicin (Rif) are the key first-line antituberculosis drugs, and resistance to these drugs i.e., MDR-TB, is likely to result in treatment failure and poor clinical outcomes. The present study was done to compare the performance of line probe assay test (GenoType® MTBDRplus) with liquid culture (MGIT 960) system for the detection of resistance to first-line drugs.
 
 Materials and Methods: We estimate the performance of LPAs to BACTEC MGIT 960 system for susceptibility testing of bacterial resistance to first-line drugs: rifampicin (RIF), isoniazid (INH).
 
 Results: We performing Drug susceptibility testing (DST), 219/258 MTB cultures were viable after subculture the results of DST using the MGIT 960 system were compared to those obtained by line probe assay. LPA detected a total 46/258 (17.81%) samples as drug resistant, of which 35/258 (13.70%) were resistant to both rifampicin and isoniazid (MDR), 6/258 (2.28%) were rifampicin mono?resistant samples and 11/258 (4.11%) were isoniazid mono?resistant. Out of the culture?positive samples (219), LPA detected 39/219 (17.83%) as drug?resistant, of which 31/219 (14.2%) were resistant to both rifampicin and isoniazid, 5/193 (2.08%) were rifampicin mono?resistant and 8/219 (3.7%) were isoniazid mono?resistant.
 Conclusion: Drug resistant TB poses an enormous threat to TB control programs worldwide. Effective treatment of MDR-TB is very expensive, particularly in middle income countries such as India.
Highlights
Introduction and AimIndia has the uppermost trouble of Multidrug resistant tuberculosis (MDR-TB) is a major challenge controlling resistance, reducing transmission and improving handling outcomes in multidrug resistance (MDR)-TB patients is dependent on susceptibility testing
Drug resistant TB poses an enormous threat to TB control programs worldwide
BACTEC MGIT 960, a rapid liquid phenotypic Drug susceptibility testing (DST) method, is used in frequent laboratories to test for resistance to first-line drugs
Summary
Introduction and AimIndia has the uppermost trouble of Multidrug resistant tuberculosis (MDR-TB) is a major challenge controlling resistance, reducing transmission and improving handling outcomes in MDR-TB patients is dependent on susceptibility testing. With a population of around 1.24 billion, India is the largest country in the South East Asian region It ranks first among the high TB burden countries which contributed 26% of the estimated global incident TB cases in 2012 and among the 27 multidrug-resistant TB (MDR-TB) high-burden countries worldwide, contributing to 21% of all MDR-TB cases estimated among notified cases [2]. The GenoType MTBDR plus assay is a commercially available Line Probe Assay (LPA) that allow specific gene markers associated with RIF and INH resistance to be detected.The GenoType ® MTBDRplus evaluation is a molecular PCR-based extension and reverse blotting evaluate that employs accurate probes hybridized to nitrocellulose strips to recognize tuberculosis and its resistance to rifampicin (RIF) and isoniazid (INH) drugs [6]. During this study was appearance of molecular line probe assay to a MGIT 960 system for the detection of resistance to Anti-TB drugs
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