Estimation of Genotoxic Effects from GSM Base Stations on Four Successive Generations (F1 F4) of Albino Mice, Mus Musculus Using RAPD Bands and Micronucleus Assay

Abstract

The levels of radiofrequency radiations from the telecommunication base station and its potential genotoxic effects on DNA content and micronucleus induction in four generations of albino mice, Mus musculus were carried out. The level of radiofrequency radiations around the GSM base station ranged between 1.3 - 2.0⇥ 10 3 µW/m 2 respectively. The genotoxic effects of radiofrequency radiation on four mice generations were based on random amplified polymorphic DNA (RAPD) and gel electrophoresis, results showed gradual depreciation in DNA band intensity of exposed mice across generations when compared to control groups. The micronucleus assay using acridine orange stain showed increased in micronucleus count and cytotoxic effects on exposed mice being significantly (PCE/NCE P<0.05) higher when compared to the control . Statistical analysis also revealed that significant genotoxic effects were induced (p<0.05) in exposed mice compared with control over the four generations,the results indicated that the RAPD and micronucleus assay could be a suitable bio-molecular marker for the detection of potential long-term genotoxic effects of radiofrequency radiations on exposed mammalian organisms.