Abstract
The current study conducted to detect the genetic diversity between four genetic groups of Kurdish local chicken using RAPD-PCR technique. Ten random markers used to amplified DNA were selected for genotyping the four lines. One hundred twenty four polymorphic bands were amplified, the bands size ranged between (2500 and 100 bp). The primer (OPA-05, OPA-18) produced maximum number of polymorphic bands, while primers named (OPA-16) produced minimum number of polymorphic bands. The (WNFS) genetic group showed the highest number of amplified fragments (55) for both male and female (30), (25) respectively. While (BBN) genetic group showed the lowest number of amplified fragments (20) for both male and female (7), (13) respectively. According to the results obtained from the current study, it can be conclude that the four genetic groups differ genetically. Moreover the results will help the breeders to study new selection strategies between the four genetic groups.
Highlights
The evolution of genetic science during the past decades, and knowledge of the impact of environment factors contribute to the emergence of new adapted poultry strains
The detected polymorphisms were varied among the four genetic groups
The (WNFS) genetic group showed the highest number of amplified fragments [55] for both male and female [30, 25] respectively
Summary
The evolution of genetic science during the past decades, and knowledge of the impact of environment factors contribute to the emergence of new adapted poultry strains. Several countries have categorized their livestock [1, 2, 3] in order to satisfy their genetic characteristics and know what genes they have. The progress of genetic science especially by development of molecular techniques, it has been possible to make selection [4], Identification [5], characterization [6], and genetic improvement [7] of livestock. Iraq is one of the countries that have wide verities of livestock animals. More over [25] studied the polymorphism of genetic lines by using RAPD markers. The method named Randomly Amplified polymorphic DNA (RAPD) was used to characterize several organisms [26] genome based on whole genom [27], and chromosome level [28]
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