Estimation of Ganciclovir Drug by Oxidative Coupling Reactions with NBS as an Oxidant

Abstract

A versatile and simple spectrophotometric technique has been presented for the detection of Ganciclovir (GANCICLOVIR) in pharmaceutical formulations. The oxidation method included mixing an excessive amount of N-bromosuccinimide (NBS) with Ganciclovir in an acidic solution, then calculating the amount of unreacted NBS using methyl blue at \(\lambda\) 610 nm. With a superior molar absorption of \(3.39 \times 10^{-3}\) L mol\(^{-1}\) cm\(^{-1}\), the amount of NBS interacting with the colorant is equal to the amount of the drug. The Sandell's sensitivity index was 0.169 g cm\(^{-2}\), the Beer-Lambert law scale was 1-35 \(\mu\)g mL\(^{-1}\), and the recovery of the concentration was 99% with a relative standard deviation of 0.01%. The technique was successfully used to measure the drug content in pharmaceutical formulations. The precise quantification of Ganciclovir, a highly effective antiviral medication, is of considerable significance in the fields of pharmaceutical analysis and clinical practice. The objective of this study was to establish a dependable and effective approach for the quantification of Ganciclovir via an oxidation reaction employing N-bromosuccinimide (NBS) as the oxidizing agent.