Abstract
Institute of Research and Development, Gujarat Forensic Sciences University,Near Police Bhavan, Sector 18A, Gandhinagar 382007, IndiaThe present study describes the estimation of DNA damage by using gold nanoparticles (AuNPs)as probe. DNA damage has been studied in various organisms by a number of methods. Thesemethods have several limitations like time, cost and require sample preparation. Thus, we havedeveloped a simple, cost effective method for estimation of DNA. AuNPs were used to measurepure and degraded DNA in a mixture by UV-spectroscopy and spectroÂuorometry. It is interesting tonote that the absorption of AuNPs increases, while Âuorescence intensity decreases on addition ofincreasing amount of degraded DNA. The study suggests that in both spectroscopic determinations,the results obtained for DNA degradation patterns were in linear range, which will deÂnitely prove tobe an effective tool in molecular biology to point out the nature of DNA damage, quantify damageand repair processes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
