Abstract
Institute of Research and Development, Gujarat Forensic Sciences University,Near Police Bhavan, Sector 18A, Gandhinagar 382007, IndiaThe present study describes the estimation of DNA damage by using gold nanoparticles (AuNPs)as probe. DNA damage has been studied in various organisms by a number of methods. Thesemethods have several limitations like time, cost and require sample preparation. Thus, we havedeveloped a simple, cost effective method for estimation of DNA. AuNPs were used to measurepure and degraded DNA in a mixture by UV-spectroscopy and spectrouorometry. It is interesting tonote that the absorption of AuNPs increases, while uorescence intensity decreases on addition ofincreasing amount of degraded DNA. The study suggests that in both spectroscopic determinations,the results obtained for DNA degradation patterns were in linear range, which will denitely prove tobe an effective tool in molecular biology to point out the nature of DNA damage, quantify damageand repair processes.
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