Abstract

Abstract Improved hydrolytic and analytical techniques show that more epinephrine, and norepinephrine is excreted in conjugate form than has previously been reported. An improved, relatively simple analytical procedure is described for these conjugates. It is shown that diet, apart from bananas, has little influence on the excretion of the conjugates. It is suggested that the estimate of conjugated epinephrine and norepinephrine be included in all urinary excretion studies of catecholamine metabolites in which comprehensive metabolic patterns are to be determined. The assay may be of special value when the differential effect of exogenous substances on norepinephrine and epinephrine metabolism is under investigation and in the study of the effect of pharmacological agents interfering with the ordinary pathways of catecholamine metabolism.

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