Abstract
The present investigation was undertaken to estimate contents of bioactive phenolic compounds in A. pindrow aerial parts using TLC densitometry. Maltol, p-coumaric acid, p-hydroxy benzoic acid, protocatechuic acid and gallic acid were resolved in TLC of methanol extract of plant using solvent systems – chloroform:methanol (9.7:0.3; scanned at 276 nm), toluene:diethyl ether (1:1; scanned at 320 nm), chloroform:methanol:glacial acetic acid (19:1:1; scanned at 266 nm), chloroform:methanol:formic acid (9:1:1; scanned at 295 nm) and chloroform:methanol:acetonitrile: formic acid (12:3:3:2; scanned at 292), respectively. The developed methods for estimation of phenolic compounds in plant were validated as per ICH guidelines. The contents of maltol, p-coumaric acid, p-hydroxy benzoic acid, protocatechuic acid and gallic acid in plant were found to be 0.10280±0.00002, 0.00860±0.00000, 0.00780±0.00001, 0.10030±0.00003 and 0.06440±0.00002% w/w, respectively. On the basis of validation parameters, the developed TLC densitometric methods for estimation of phenolic compounds in the plant were found to precise, accurate and specific.
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