ESTIMATION OF ATOSIBAN ACETATE IN PARENTERALS BY RP HPLC

Abstract

A simple, precise, rapid and accur a te reverse phase HPLC method was developed for the estimation of Atosiban Acetate in dosage form. A n XTerra MS C18 analytical column ( 250 x 4.6 mm , 5 μm partical size ) with mobile phase consisting of mixture of buffer 0.0 3 M Potassium Dihydrogen Orthophosphate in water and pH adjusted to 3.20 with o rtho - phosphoric acid and acetonitrile in the gradient program was used. The flow rate was 1.0 mL /min an d the effluents were monitored at 2 2 0 nm . The retention time was 1 1.3 min . The detector response was linear in the concentration of 15 - 225 mc g/ mL . The respective linear regression equation being y = 1286.9 x - 1290 . 8 . The limit of detection (LOD) and limit of quantification (LOQ) for Atosiban Acet ate were found to be 1.5 mcg/mL and 4.5 mcg/mL respectively . The percentage ass ay of Atosiban Acetate was 99. 6 %. The method was validated by determining its accuracy, precision and linearity . The results of the stu dy showed that the proposed RP - HPLC method is simple, rapid , precise and accurate, which is useful for the