Abstract
Antioxidant activity of 19 different mixed herbal infusion as it was calculated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays was correlated using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics. The spectral region 1538- 843 cm-1 in 2nd derivative mode and the partial least squares (PLS) regression were used. The correlation coefficient, the root-mean-square error of calibration (RMSEC) and the root-mean-square error of prediction (RMSEP) for the DPPH assay were 0.97, 130 and 118 respectively. The corresponding values for the ABTS assay were 0.99, 148 and 121. The above results show that it is possible to estimate the antioxidant activity according to DPPH and ABTS assays of different mixed herbal infusions using ATR-FTIR spectroscopy. Furthermore the proposed methods are simple, rapid and economical.
Highlights
Free radicals are products of oxygen and nitrogen that come from the metabolic paths
The aim of this work is to develop an alternative methodology for the antioxidant activity estimation, according to DPPH and ABTS assays, of various herbal infusions for routine analysis
Antioxidant activity according to DPPH and ABTS assays The antioxidant activity of the 19 herbal infusions was determined using the DPPH and ABTS assays (Tables 1 and 2)
Summary
Free radicals are products of oxygen and nitrogen that come from the metabolic paths. The excessive amount of these free radicals is the cause of chronic diseases in human (Dhar et al, 2012; Aktumsek et al, 2013). Antioxidants in food play an important role for human’s health since the scientific community has proven that they can reduce the risk of chronic diseases such as cancer, heart disease and Alzhemier’s disease (Aktumsek et al, 2013; Rautiainen et al, 2013; Saeidnia and Abdollahi, 2013). Phenolic compounds are responsible for the antioxidant activity and the main sources of them are fruit, vegetables, legumes, fats/oils and herbal infusion (Dhar et al, 2012; Reutenbach and Venter, 2013; Lou et al, 2014). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Brand-Williams and others 1995) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods (Re et al.,1999) were used widely
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