Abstract
A spectrophotometric assay for the adenine nucleotides using AMP deaminase, myokinase (MK) and apyrase has been examined and found to give erroneously high estimates of AMP, ADP and ATP in rat brain extract. The AMP deaminase preparation used is liable to contain creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities. However, it is suggested that the discrepancy may be due to the presence of yet another enzymic contaminant. Some aspects of nucleotide extraction from brain tissue are investigated.
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