Estimation of Active Compounds Quantity from Pharmaceuticals Based on Ginkgo biloba

Ramona Oana Gunache (Roșca),Constantin Apetrei

doi:10.3390/chemosensors8040110

Ramona Oana Gunache (Roșca), Constantin Apetrei

Open Access

https://doi.org/10.3390/chemosensors8040110

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Journal: Chemosensors	Publication Date: Nov 4, 2020
Citations: 5	License type: CC BY 4.0

Affiliation: "Dunarea de Jos" University of Galati

Abstract

Ginkgo biloba is one of the most important sources of active compounds, mainly flavonoids and phenolic compounds. Due to its importance related to pharmaceutical practice, the making of a qualitative and quantitative method for the detection and quantification of active compounds from Ginkgo biloba pharmaceutical products is desirable. In this study, the content of biological active compounds from Ginkgo biloba products was estimated using cyclic voltammetry. The electrochemical determination of active compounds was carried out by using a screen-printed carbon electrode modified with carbon nanotubes. The studies regarding parameter optimization were made using solutions containing potassium ferrocyanide and catechol, respectively. In both cases, the redox processes of studied compounds was observed, which were controlled by the diffusion phenomenon. We analyzed two pharmaceutical products containing Ginkgo biloba, a RX product (recipe medicine requires a medical prescription to be dispensed) and an OTC (Over-The-Counter, which can be obtained without a prescription) product. The cyclic voltammograms of the two products showed two redox processes due to the antioxidant properties of the products. It was found that the RX product had a greater content of active compounds compared to the OTC product. Therefore, the voltammetric method has great utility for the determination of compounds with redox properties from pharmaceutical products containing Ginkgo biloba.

Highlights

Ginkgo biloba is one of the main sources of active substances such as flavonoids and phenolic compounds [1]
Numerous analytical methods have been developed for the determination of the antioxidant capacity such as chemical methods, spectrophotometric (Folin–Ciocalteu method, determination of antiradicalic capacity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) or 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)), and electrochemical methods based on cyclic voltammetry [5,6]
In this study, a series of pharmaceutical products based on Ginkgo biloba were analyzed by cyclic voltammetry and the antioxidant properties were correlated with the antioxidant capacity determined by the DPPH assay

Summary

Introduction

Ginkgo biloba is one of the main sources of active substances such as flavonoids and phenolic compounds [1]. Due to its importance in pharmaceutical practice, a qualitative and quantitative method has been searched for the detection and quantification of active compounds from Ginkgo biloba based products [4]. In pursuing this goal, numerous analytical methods have been developed for the determination of the antioxidant capacity such as chemical methods, spectrophotometric (Folin–Ciocalteu method, determination of antiradicalic capacity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) or 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)), and electrochemical methods based on cyclic voltammetry [5,6]. The pharmaceutical products based on Ginkgo biloba that are produced with antioxidant properties can be analyzed with electrochemical methods [7]. These methods can be used in routine analysis because they are fast, easy to carry out, and inexpensive [8]

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