Abstract

Conditions were optimized for the quantitative extraction and isolation, by reversed-phase adsorption, of a saponin, from the common or purple starfish, Asterias vulgaris, and for the subsequent solvolytic release and quantitation of a steroid aglycone, 5α-pregn-9(11)-ene-3β,6α-diol-20-one. With the method developed, the abundance of the aglycone was found to vary seasonally and was greatest from April to June at two locations on the Atlantic coast of Nova Scotia. When several locations were examined during October, the abundance of the aglycone was also found to vary geographically.

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