Abstract
Nucleoside proton affinity was determined using Cooks' kinetic method. An ion trap mass spectrometer coupled with an external electrospray source was used to form proton-bound homo- and heterodimers from modified uridines and amino acids at low effective temperature. The experimental proton affinity ( PA) values obtained show the effects of structural changes in nucleosides on PA values (i.e. U < dU and 5IdU < dU < dT). The calculated values are consistent with those reported by tandem sector instruments. In this way, an hypothesis on the interaction site of the heterodimers is proposed: the amino groups are likely to interact with the proton (in the dimer) as expected from its solution behavior.
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