Abstract
Number of presumptive tests is there for detection of blood in forensic science. But problems arise in choosing the presumptive test that gives the least false positive result. Difficulties arise due to the small quantity of blood found, so to prevent damage of the sample appropriate reagent is required for the detection of blood. Blood, artificial blood, fake blood, and some false positive-giving substances (including commercial products such as Bleach, Enamel paint, Sauce, and Betadine, chemicals such as copper sulphate, ferrous oxide, and potassium permanganate, vegetables/cereals/fruits such as cauliflower, strawberries, red kidney beans, and rust) were used in these studies. Presumptive tests like Kastle-Meyer, Tetra methyl benzidine, leucomalachite green, BLUESTAR® FORENSIC and Fluorescein test were used. To overcome this problems, comparison of these test reagents are done. In comparison to all other test reagents, Lecomalachite green has the highest accuracy, followed by BLUESTAR® FORENSIC, Fluorescein, Kastle-Meyer, and Tetramethylbenzidine test, which has the lowest accuracy.
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