Estimating the post-mortem interval (I): The use of genetic markers to aid in identification of Dipteran species and subpopulations

Abstract

Insect evidence can be used to estimate the post-mortem interval (PMI) of a discovered corpse. They can also indicate any post-mortem movement of a body. To use insect evidence accurate identification is essential. This study examined the use of mitochondrial cytochrome oxidase I markers to differentiate Calliphora vicina and Calliphora vomitoria (Diptera:Calliphoridae). The work also explored the use of nuclear DNA markers to distinguish UK populations of these species. DNA extraction and subsequent amplification of these markers in various lifecycle stages, adult body parts and burnt/decomposing larvae ensured that the markers could be used with any insect evidence presented to the entomologist.