Estimating the level of amino acids in patients with liver disease by amino acid analyzer

Abstract

Background: Amino acid identification can be a reliable approach to noninvasive diagnosis of liver disease. This is because liver disease can be a cause of impaired amino acid metabolism. Therefore, a method for determining amino acids in blood that is applicable for clinical purposes is essential. Objectives: The aim of this study was to find differences in amino acid levels between patients with liver disease and a control group. Material and Methods: Samples of peripheral venous blood were obtained from a group of patients with liver disease (n = 40 women at an average age of 37-70 years and 40men at an average age of 35-70 years) and control group (n = 40 women at an average age of 35- 70 years and 40 men at an average age of 35 - 70 years). Before separation, The amino acids were derived using orthophalenealdehyde (OPA).For separation, a reverse phase shaft was used. The flow was monitored with a fluorescence detector. Results. There were statistically significant differences in the concentrations of some amino acids between patients and the control group, as well as between women and men. Relationships between some amino acids, markers of liver blood tests, and lipid metabolism were observed.