Estimating the Length Distributions of Genomic Micro-satellites from Next Generation Sequencing Data

Abstract

Genomic micro-satellites are the genomic regions that consist of short and repetitive DNA motifs. In contrast to unique genome, genomic micro-satellites expose high intrinsic polymorphisms, which mainly derive from variability in length. Length distributions are widely used to represent the polymorphisms. Recent studies report that some micro-satellites alter their length distributions significantly in tumor tissue samples comparing to the ones observed in normal samples, which becomes a hot topic in cancer genomics. Several state-of-the-art approaches are proposed to identify the length distributions from the sequencing data. However, the existing approaches can only handle the micro-satellites shorter than one read length, which limits the potential research on long micro-satellite events. In this article, we propose a probabilistic approach, implemented as ELMSI that estimates the length distributions of the micro-satellites longer than one read length. The core algorithm works on a set of mapped reads. It first clusters the reads, and a k-mer extension algorithm is adopted to detect the unit and breakpoints as well. Then, it conducts an expectation maximization algorithm to approach the true length distributions. According to the experiments, ELMSI is able to handle micro-satellites with the length spectrum from shorter than one read length to 10 kbps scale. A series of comparison experiments are applied, which vary the numbers of micro-satellite regions, read lengths and sequencing coverages, and ELMSI outperforms MSIsensor in most of the cases.