Abstract
The Kato-Katz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials pertaining to intestinal schistosomiasis and soil-transmitted helminthiasis. However, the sensitivity of the technique is low, particularly for the detection of light-intensity helminth infections. Examination of multiple stool samples reduces the diagnostic error; yet, most studies rely on a single Kato-Katz thick smear, thus underestimating infection prevalence. We present a model which estimates the sensitivity of the Kato-Katz technique in Schistosoma mansoni and hookworm, as a function of infection intensity for repeated stool sampling and provide estimates of the age-dependent ‘true’ prevalence. We find that the sensitivity for S. mansoni diagnosis is dominated by missed light infections, which have a low probability to be diagnosed correctly even through repeated sampling. The overall sensitivity strongly depends on the mean infection intensity. In particular at an intensity of 100 eggs per gram of stool (EPG), we estimate a sensitivity of 50% and 80% for one and two samples, respectively. At an infection intensity of 300 EPG, we estimate a sensitivity of 62% for one sample and 90% for two samples. The sensitivity for hookworm diagnosis is dominated by day-to-day variation with typical values for one, two, three, and four samples equal to 50%, 75%, 85%, and 95%, respectively, while it is only weakly dependent on the mean infection intensity in the population. We recommend taking at least two samples and estimate the ‘true’ prevalence of S. mansoni considering the dependence of the sensitivity on the mean infection intensity and the ‘true’ hookworm prevalence by taking into account the sensitivity given in the current study.
Highlights
Soil-transmitted helminthiasis (STH) and schistosomiasis are two of the most prevalent neglected tropical diseases with more than 1 billion and over 250 million people affected worldwide, respectively [1, 2]
The three studies are from different hookworm transmission settings with observed prevalence of 11.4% and mean infection intensity of an infected individual of 396 eggs per gram of stool (EPG) for Azaguie, 35.4% and 331 EPG for Zouatta, and 59.0% and 283 EPG for Fagnampleu
The estimated mean infection intensity does not significantly differ from one study to another and mean estimates ranged from 220 EPG to 262 EPG
Summary
Soil-transmitted helminthiasis (STH) and schistosomiasis are two of the most prevalent neglected tropical diseases with more than 1 billion and over 250 million people affected worldwide, respectively [1, 2] Their collective global burden is 6 million disability-adjusted life years [3], with school-aged children at the highest risk of associated morbidity. Diagnosis relies on the Kato-Katz technique [10], i.e., counting of helminth eggs in a small amount of stool This approach, has a low, setting-dependent sensitivity, which is governed by variation in day-to-day production of eggs per worm, non-random distribution of eggs within a stool sample, decay of eggs in the sample due to methods and duration of the experimental procedure, transportation, and storage [11,12,13,14,15]. The comparison of studies that employed different sampling efforts, which is necessary for monitoring progress of control programs, is hampered
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
