Abstract
Open-system respirometry offers a practical approach for measuring metabolic rates of fish cultured at high densities in uncovered raceways. Central to this methodology is analysis of a dynamic mass-balance on oxygen supply and demand. Here, we present a validated mass-balance equation, describe minimally disruptive procedures for estimating its parameters, and illustrate its use in estimating the oxygen-uptake rate of fish as a group, in real time and under actual production conditions.
Highlights
Oxygen respirometry is the dominant technique for estimating aerobic metabolism of fish and other water-breathing animals
The reaeration rate K is a measure of how effectively the raceway is resupplied with oxygen via aeration or injection of oxygen
At the moment of interest, metabolic rate of the fish per gram body weight was 40,000/175,050 = 0.23 mg 0/(g*h). Is this value right or wrong? It can only be stated that this number is consistent with results from closed-system respirometry (Forsberg and Neill 1998)
Summary
Oxygen respirometry is the dominant technique for estimating aerobic metabolism of fish and other water-breathing animals. The rate of oxygen-uptake by a fish in a closed or semi-closed chamber is presumed to be equivalent to the rate of oxygen disappearance from the water contained in or flowing through the chamber. The equivalency may or may not be adjusted for disappearance or appearance of oxygen in a "blank" control chamber, attributable to microbial activity. Static respirometers have only the water movement necessary to assure mixing. International Journal of Recirculating Aquaculture, Volume 4 25 and adequate irrigation of the oxygen electrode; active respirometers are intended for measuring oxygen-uptake rate in fish forced to swim at constant speed against a water current. Springer and Neill (1988) have described the development of computer-automated respirometry Cech (1990) has provided a thorough review of conventional respirometry. Springer and Neill (1988) have described the development of computer-automated respirometry
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