Abstract
We compared denitrification rates obtained using an adaptation of the acetylene block technique to rates estimated from benthic flux nutrient stoichiometry in the subtidal sediments of Tomales Bay, California (USA). By amending whole cores with acetylene and saturating nitrate concentrations, we obtained potential denitrification rates, which ranged between 4 and 30 mmol N m−2 d−1. We determined the apparent Michaelis constant (Kapp) and the maximum potential rate (Vmp) of the denitrifying community and used these constants in a rectangular hyperbola to estimatein situ denitrification rates. Both the Kapp and Vmp of the denitrifying community exhibited significant variation over both depth in the sediment column and time of sampling.
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