Estimates of Motor Neuron Excitability During Postural Sways in Lower Leg Muscles

Abstract

Motor system investigations are often performed under isometric conditions, but real‐world motor behaviors are seldom isometric. During tasks where isometric force or torque measurements are not feasible (i.e. during unconstrainedconditions), EMG is often used as a surrogate of the level of muscle activity. However, the linear relationship between EMG and force generation breaks down during non‐isometric contractions. It is not clear how this affects investigators’ ability to discriminate motor units using state‐of‐the‐art high‐density surface EMG arrays. Additionally, the paired motor unit analysis technique, which generates an estimate of the intrinsic excitability of a ‘test’ motor unit by determining discharge rate hysteresis (ΔF), has not been evaluated in unconstrained conditions. Consequently, we asked if 1) visual feedback provided by the subject’s own EMG activity allow for subjects to adequately perform triangular ramp contractions in constrained conditions and 2) if the EMG visual feedback is provided in unconstrained conditions, are ΔF values in subjects similar to those in the constrained condition? We hypothesized that during voluntary isometric ramp contractions, ΔF will be similar regardless of the feedback source. We further hypothesized that standing postural adjustments using EMG feedback in unconstrained conditions will produce similar ΔF values to those calculated during the isometric ramps. We expected that although the mean values across conditions will be similar, the inter‐subject coefficient of variation of ΔF will be greater in the less constrained condition. Accordingly, surface EMG using high‐density array electrodes was collected from five subjects under three conditions: 1) seated in a dynamometer in the isometric mode with torque feedback, 2) in the isometric dynamometer with filtered EMG feedback, and 3) during postural adjustments while standing freely with EMG feedback. In agreement with our hypothesis, we found that ΔF values across all three conditions were similar. In the constrained conditions with either torque or EMG feedback ΔF was 3.10 ± 0.70 imp/s and 3.40 ± 0.75 imp/s, respectively; during the unconstrained case with EMG feedback, ΔF was 3.08 ± 0.73 imp/s (all n = 15, mean ± SE). Contrary to our expectations, the coefficient of variation was not greater in the unconstrained standing condition. These results demonstrate that real‐time EMG feedback is sufficiently similar to torque feedback during both isometric and near‐isometric, unconstrained contractions, suggesting that detailed investigations of motor unit activity may be expanded to more functional movements and used relatively easily in more clinical settings.