Abstract
In many cases evaluation of chlorophyll and nitrogen content in plants need to destructive methods, more time and organic solvents. Application of chlorophyll meters save time and resources. The aim of this study was estimating of chlorophyll and nitrogen content in Asian pear leaves using non-destructive method and rapid quantification of chlorophyll by chlorophyll content meter (CCM200). This study was conducted on 8 years old Asian pear trees during June 2008 in Tehran, Iran. To develop our regression model, the chlorophyll meter data were correlated with extracted chlorophyll and nitrogen content data obtained from DMSO and Kejeldal methods, respectively. The results showed that, there was positive and linear correlation between CCM-200 data and chlorophyll a (R²=0.7183), chlorophyll b (R²=0.8523), total chlorophyll (R²=0.90), and total nitrogen content (R²=0.76) in Asian pear leaves. Thus, it can be concluded that, CCM-200 can be used in order to predict both chlorophyll and nitrogen content in Asian pear leaves.
Highlights
Nitrogen is one of the most important macro nutrients and plays critical role in vegetative growth, flowering and fruit development (Shaahan et al, 1999)
Changes in chlorophyll content occur as a result of nutrient deficiency, especially nitrogen with consider to this point that nitrogen is leached from soil quickly
Since chlorophyll concentration is correlated with leaf nitrogen concentration, the instruments which are used to evaluate chlorophyll concentration such as SPAD-502 (Minolta, Japan) and Chlorophyll content meter CCM-200, have been designed to determine nitrogen content (Pavan et al, 2004)
Summary
Nitrogen is one of the most important macro nutrients and plays critical role in vegetative growth, flowering and fruit development (Shaahan et al, 1999). Regarding the relationship between chlorophyll content and leaf nitrogen content, chlorophyll meter can be used to improve fertilizer management (Richardson et al, 2002). A close correlations between chlorophyll meter with foliar nitrogen and total extracted chlorophyll has been reported in several fruit trees such as mango, mandarin, guava and grapevine (Shaahan et al, 1999), in other crops such tobacco leaves (Kowalczyk-Jusko and Koscik, 2002) and sugar maple (Van den Berg and Perkins, 2004).
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