Abstract
The incorporation of [1- 14C]oleic acid and [1- 14C]elaidic acid into cell lipids of the swine aortic smooth muscle cells under aerobic and anaerobic conditions was investigated. The uptake of elaidic acid was higher than that of oleic acid; however, most elaidic acid taken up by aortic smooth muscle cells was kept in an unesterified form. As compared to elaidic acid, significant amounts of oleic acid were incorporated into phospholipids, triglycerides, and cholesteryl esters under both aerobic and anaerobic conditions. More labeled oleic acid and elaidic acid was esterified into triglycerides under anaerobic than aerobic conditions. Of individual phospholipid fractions studied, both labeled oleic and elaidic acids were incorporated predominantly into phosphatidylcholine fraction.
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