Abstract
Triicosapentaenoyl (EPA-TG) and tridocosahexaenoyl glycerols (DHA-TG) were prepared by lipase TOYO (Chromobacterium viscosum) -catalyzed esterification. The FFAs (EPA and DHA) reacted with glycerol in the presence of a lipase solution to give a mixture of acylglycerols whose unreacted hydroxyl groups were subsequently esterified by the corresponding acids in the presence of the immobilized lipase on celite. 100 % of DHA-TG and 92 % of EPA-TG were obtained under optimal conditions.
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