Esterase mediated resistance against synthetic pyrethroids in field populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in Punjab districts of India

Abstract

Detection of resistance levels against cypermethrin and deltamethrin, the most commonly used synthetic pyrethroids (SP), in Rhipicephalus (Boophilus) microplus collected from thirteen districts of Punjab (India) was carried out using adult immersion test. The regression graphs of probit mortality of ticks plotted against log values of concentrations of drugs were utilized for the determination of slope of mortality, lethal concentration for 50% (LC50), 95% (LC95) and resistance factor (RF). On the basis of the data generated on variables (mortality, egg mass weight, reproductive index and percentage inhibition of oviposition) the resistance levels were categorized. Against cypermethrin RFs of 1.48–11.22 were recorded in 12 isolates whereas, one isolate was susceptible. Resistance factors against deltamethrin were 2.4–38.54 and all 13 isolates were found to be resistant. Quantitative analysis of general esterase activity (measured by the production of the metabolite naphthol) revealed a range of 3.34±0.30–13.75±1.33 and 1.31±0.15–8.09±0.68μmol/min/mg protein for α and β-esterase activity, respectively in different field isolates. Further, multiple pairwise comparisons of the mean values with susceptible strain (Tukey, P=0.05) revealed significant elevated levels of both α-esterase and β-esterase in nine tick isolates resistant to both deltamethrin and cypermethrin. The data generated on acaricide resistant status and esterase mediated mechanism in ticks will help in formulating tick control strategy for the region.