Esterase D: Simultaneous electrophoretic determination of the three common allozymes (ESD 1, 2, 5)

Abstract

AbstractA simple and time‐saving electrophoretic method for the determination of the 6 common phenotypes of red cell esterase D (ESD) is presented. Using a continuous buffer system composed of malic acid, the separation of the allozymes is accomplished at pH‐values between 5.35–5.45 by thin‐layer agarose gel electrophoresis in 4 h. Until now, a combination of electrophoresis and isoelectric focusing was required to obtain the same results. The economical advantage as well as the modest requirements concerning laboratory equipment are evident.