Establishment of Yan-Zhang Staging of Digestive Tract Magnetic Compression Anastomosis in a Rat Model

Abstract

INTRODUCTION: Magnetic compression anastomosis (magnamosis), is a safe and feasible method for gastrointestinal anastomosis. However, the pathological process involved in magnamosis of the digestive tract has not been investigated. This study aimed to establish the stages of digestive tract magnamosis in a rat model. METHODS: Eighty-four Sprague-Dawley albino rats (200-250 g) were randomly divided into 14 groups (n = 6 per group). All rats underwent colonic magnamosis. Starting from postoperative day (POD) 1, 1 group of rats was sacrificed every other day to obtain the specimens. Burst pressure at the anastomotic site of each specimen was examined. Gross and histological examination of the anastomotic site of each specimen was performed to establish the stages of the digestive tract magnamosis. RESULTS: Colonic magnamosis was successfully performed in all rats and the mean anastomosis time was 5.62 ± 0.91 minutes. The postoperative survival rate was 100%. The lowest anastomotic burst pressure was 78.33 ± 3.44 mmHg on POD3. The anastomotic burst pressure gradually increased and stabilized on POD21. Macroscopic and histological examination showed that the anastomotic mucosal and serosal layer did not heal on POD1. The serosal layer of the anastomosis healed by adhesion on POD3, and the mucosal layer began to heal on POD3-11 and was established by POD21. CONCLUSION: According to the anastomotic bursting pressure, gastrointestinal magnamosis can be divided into the magnetic maintenance, fragile, strengthening, and stable period, which correspond to serosal adhesion formation, serosal healing, mucosal healing, and stereotyping, respectively, based on histological changes.