Establishment of White-Backed Planthopper Cell Lines.

Abstract

The first continuous cell line of leafhopper was established over 50years ago. Since then, leafhopper cell monolayers have been used extensively to assay the infectivity of plant viruses that multiply in their insect vectors and to elucidate the viral determinants for virus transmission via insects. We have established continuous insect cell lines of three rice planthoppers, which have been used to study the mechanisms for replication and spread of rice viruses. The notable advantage of the vector cell monolayer system is that it can reach a uniform infection rate of 100% of the cells in the culture inoculated with diluted viruses, and thus allows for synchronous virus multiplication. The self-propagative nature of leafhopper and planthopper cell lines under favorable conditions ensures the system both dynamic and stable for viral infection. The vector cell monolayer systems and molecular probes, along with reliable traditional methods, certainly facilitate studies on interactions between plant viruses and insect vectors at molecular and cellular levels.