Establishment of the cultured model of microencapsulated human gastric carcinoma cells SGC-7901

Abstract

Objective To establish the cultured model of microencapsulated human gastric carcinoma (HGC) cell line SGC-7901.Methods The SGC-7901 cells were cultured and encapsulated in APA microcapsules using the prepared electrostatic droplet generator for 21 days.The level of proliferation and metabolism of the microencapsulated HGC cells were detected by using methyl thiazol tetrazolium ( MTT),and glucose and lactic acid concentrations were determined in the supernatant.Microencapsulated HGC cells were stained with hematoxylin and eosin (HE),and the expression of bromodeoxyuridine ( BrdU),vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) gene was detected by using immunocytochemistry on the 7th,14th and 21st day.Monolayer cultured cells served as controls.Results On the 14th day,the relative number of the microencapsulated cells was no longer increased and decreased slightly on the next few days.Glucose was consumed and its concentration was decreased to 10 mmol/L,and lactic acid was generated and its concentration was increased to 100 mol/L in microencapsulated HGC cells on the 20th day.The expression of PCNA and VEGF gene could be detected in SGC7901 cells.The expression of BrdU,PCNA and VEGF was still detected in the microencapsulated SGC9701 cells on the 7th and 14th day.On the 21st day,the expression of Brdu and PCNA was found on the outer layer of spheroid,and that of VEGF gene was detected within the whole spheroid.Conclusion The cultured model of microencapsulated HGC SGC9701 had been established,and gene expression was stable. Key words: Gastric carcinoma; Microencapsulation; Bromodeoxyuridine; Proliferating cell nuclear antigen; Vascular endothelial growth factor