Abstract
Sorghum is one of the world's important crops after wheat, rice, maize, and barley. Although the sorghum genome had been well-sequenced, genetic breeding and functional genome research in sorghum cultivar BTx623 is still limited due to the lack of efficient and stable genetic transformation and regeneration system in sequencing. In this study, the immature embryos of sorghum genome-sequencing cultivar BTx623 was used as the explants material, and the bar gene resistant to phosphoglyphosate was used as the screening marker for Agrobacterium -mediated sorghum genetic transformation. By screening the adaptability of callus to different concentrations of phosphoglyphosate, the appropriate concentration of phosphoglyphosate in the genetic transformation of sorghum cultivar BTx623 was determined to be 2.5 mg/L, and BTx623 immature embryo was used as explants to obtain resistant callus. After screening, regenerated plants were obtained by treating resistant callus with 0.0067 mg/L ZNC in regeneration medium. Therefore, this study successfully obtained resistant callus and regenerating plants, and established a genetic transformation and regeneration system in sorghum cultivar BTx623, which may have great significance for functional genome research and genetic breeding in sorghum.
Highlights
Sorghum is one of the world's important crops after wheat, rice, maize, and barley
The immature embryos of sorghum genome-sequencing cultivar BTx623 was used as the explants material, and the bar gene resistant to phosphoglyphosate was used as the screening marker for Agrobacterium-mediated sorghum genetic transformation
The current sorghum genome sequence is mainly derived from BTx623, so the lack of an effective BTx623 genetic transformation and regeneration system will hinder the development of sorghum genetic engineering and genome function research
Summary
The sorghum genome had been well-sequenced, genetic breeding and functional genome research in sorghum cultivar BTx623 is still limited due to the lack of efficient and stable genetic transformation and regeneration system in sequencing. The immature embryos of sorghum genome-sequencing cultivar BTx623 was used as the explants material, and the bar gene resistant to phosphoglyphosate was used as the screening marker for Agrobacterium-mediated sorghum genetic transformation. This study successfully obtained resistant callus and regenerating plants, and established a genetic transformation and regeneration system in sorghum cultivar BTx623, which may have great significance for functional genome research and genetic breeding in sorghum. Many studies used this method to obtain genetically transformed sorghum plants but the transformation efficiency and regeneration rate were still very low (Zhu et al, 2011). The current sorghum genome sequence is mainly derived from BTx623, so the lack of an effective BTx623 genetic transformation and regeneration system will hinder the development of sorghum genetic engineering and genome function research
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