Establishment of Resistance to Growth of Ehrlich Ascites Carcinoma in C57 Black Mice.

Abstract

By x irradiation it has been possible to alter the Ehrlich ascites carcinoma cells in such a way that their viability is lowered. The C57BL mice are consequently able to control the growth or these altered cells. Simultaneous with the slowed rate of growth of the cells the host produces a resistance to the cancer cell. The resistance, however, does not reach a level adequate to protect the mouse against fully viable cells until at least five injections of the altered cells have been made. Cf 50 mice receiving five to eight injections of the x-irradiated cells 47 survived a challenge of viable cancer; of 47 given a second challenge 39 survived; of 13 given a thind challenge 11 survived; of 8 given a founth chalienge all 8 survived; of 6 given ftfth, stxth and seventh challenges all 6 survived and of 2 given an eighth challenge 2 survived. Evidence for the resistance factor being an antibody is presented in the demonstration of complementfixing materials. This presently ts being evaluated further and quantitated. Oxidative and glycolytic studies of the x-irradiated cells, and of those cells showing, regression in the mouse, have a somewhat altered metabolism. There is at least amore » partial recovery of the viability of the x-irradiated cells as they grow and regress, indicated by their ability to kill mice that have not previously been given the altered cells. (auth)« less