Establishment of Pluripotent Cell Cultures to Explore Allelopathic Activity of Coffee Cells by Protoplast Co-Culture Bioassay Method.

Shinjiro Ogita,Muchamad Imam Asrori,Hamako Sasamoto

doi:10.3390/plants9091170

Abstract

We focused on the demonstration of a new pluripotent coffee cell culture system to control the growth and metabolic functions. Somatic cells in the epidermal layer of in vitro somatic embryos (SEs) of Coffea canephora expressed higher pluripotency to produce secondary SEs than primary or secondary meristematic tissue. SEs were ideal explants to selectively induce functionally-differentiated cell lines, both non-embryogenic callus (nEC) and embryogenic callus (EC). The protoplast co-culture bioassay method was used to explore allelopathic activity of these cultured coffee cells. Cell wall formation of lettuce protoplasts varied after five days of co-culture. A strong stimulative reaction was observed at lower nEC protoplast densities, whereas growth was inhibited at higher densities. The reaction of lettuce protoplasts after 12 days of co-culture was recognized as an inhibitory reaction of colony formation.

Highlights

Two primary meristems, the shoot apical meristem (SAM) and root apical meristem (RAM), located at the tip of the stem and root, respectively, are responsible for plant longitudinal growth [1]
In vitro somatic embryogenesis may be a practical model for the expression of totipotency based on the capacity of an embryogenic cell to regenerate and develop into a somatic embryo that can show the functional changes of the SAM, RAM, and cambium under certain conditions [6]
Direct somatic somatic embryogenesis embryogenesis was induced in coffee coffee leaves, and maintained maintained in the the same same medium medium

Summary

Introduction

Two primary meristems, the shoot apical meristem (SAM) and root apical meristem (RAM), located at the tip of the stem and root, respectively, are responsible for plant longitudinal growth [1]. One of the fundamental issues in plant science is to unveil the regulatory mechanisms of the series of functional changes that occur in plant meristems during their development [4]. Numerous studies have been carried out to characterize the totipotency of plant cells in terms of morphology, physiology, and molecular biology [5]. In this context, in vitro somatic embryogenesis may be a practical model for the expression of totipotency based on the capacity of an embryogenic cell to regenerate and develop into a somatic embryo that can show the functional changes of the SAM, RAM, and cambium under certain conditions [6]

Methods

Results

Conclusion