Establishment of plant regeneration system for young leaf explants of Cassia mimosoides

Yali Yang,Dadu Lin,Zhentao Liu,Shengyuan Zhang,Peiting He,Lubin Zhang,Ying Liu,Ming Zhai

doi:10.1051/e3sconf/202124503067

Yali Yang, Dadu Lin + Show 6 more

Open Access

https://doi.org/10.1051/e3sconf/202124503067

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Abstract

In the present study, a plant regeneration system was established by using young leaf explants from aseptic seedlings of Cassia mimosoides. The results showed that when the explants were inoculated on the 1/2MS medium contained 2.0 mg/L 6-BA and 0.5 mg/L NAA, high rate of callus induction and good growth status of adventitious bud, and the highest number of buds were obtained. Further studies conclusively suggested that IBA could not promote root formation effectively, but the root regeneration could be induced by NAA. However, although the rooting of regeneration buds could be induced when adventitious buds were placed into 1/2MS medium supplemented with 0.5 mg/L NAA, the rate of rooting was reduced, and the roots became shorter. Therefore, the best medium for adventitious bud regeneration using young leaf as explants in C. mimosoides was 1/2MS medium contained 2.0 mg/L 6-BA and 0.5 mg/L NAA, and the best rooting medium was 1/2MS.

Highlights

Cassia mimosoides Linn., belongs to the genus of Cassia in the legume family [1]
Young leaf explants were inoculated on 1/2MS medium contained 2.0 mg/L 6-BA and A: 0 mg/L, B: 0.5 mg/L, C: 1 mg/L NAA for induction of callus and adventitious bud in C. mimosoides for 80 days. (Bars=1 cm)
Our preliminary results suggested that when the explants were placed onto the 1/2MS medium supplemented with 2.0 mg/L 6-BA and 0.5 mg/L NAA, high callus induction rate and good growth status of adventitious bud, and the highest number of buds were gained

Summary

Introduction

Cassia mimosoides Linn., belongs to the genus of Cassia in the legume family [1]. It is mainly distributed in the provinces of Guangdong, Guangxi, Sichuan, Hunan and Fujian in China. It has the effect of clearing away heat and detoxifying, invigorating spleen and dampness, and purging, mainly used for treating many diseases such as jaundice, vomiting and diarrhea, infantile malnutrition and edema, habitual constipation and malignant boils and so on [2]. The serum containing the active components of C. mimosoides has a protective effect on liver cell injury [9]

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