Abstract
One of the two nucleoli of tetraploid PtK2 WA cells in early prophase was irradiated with an ultraviolet (UV) laser microbeam. The daughter cells that maintained the nucleolar deficiency were isolated and cloned. Five nucleolar deficient sublines of PtK2 WA were established, thus providing an experimental system to study the ribosomal gene-nucleolar organizer complex.
Highlights
Short notes 235 nucleolar deficiency were isolated and cloned
One of the two nucleoli of tetraploid PtK2 WA cells in early prophase was irradiated with an ultraviolet (UV) laser microbeam
Appropriate cells with two nucleoli in early prophasewere selected for irradiation
Summary
The purpose of this study was to establish sublines of PtK2 cells with selectively induced nucleolar deficiencies and to examine the stability of these deficiencies as well as the growth and survival of the cell populations
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