Abstract
Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase activity and their capacity to form complex embryoid bodies with rhythmically contracting cardiomyocytes. Two lines, USP-1 and USP-3, with the best in vitro characteristics of pluripotency were used in chimera-generating experiments. The capacity to contribute to the germ line was demonstrated by the USP-1 cell line. This cell line is currently being used to generate mouse models of human diseases.
Highlights
Murine embryonic stem (ES) cell lines are derived from cells isolated in vitro from the inner cell mass of blastocysts and are known to be pluripotent [1,2]
Eight independent ES cell lines were obtained from 40 blastocysts from 129/Sv mice
Cytogenetic analysis revealed a near diploid chromosome number in all but one line (A31) (Figure 1). This line had more than 90% of near tetraploid (2n = 77-80) karyotypes
Summary
Murine embryonic stem (ES) cell lines are derived from cells isolated in vitro from the inner cell mass of blastocysts and are known to be pluripotent [1,2]. The developmental potential of ES cells can be evaluated by means of in vitro tests, and especially in vivo through the formation of germ-line competent chimeras. The conditions for the differentiation of murine ES cells may be adapted to the human ES cell lines.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
