Establishment of new ELISA system for HLA-G

Abstract

s for the 29th Annual Congress of Japanese Society / Journal of Reproductive Immunology 106 (2014) 1–20 15 Different circulating levels of monocyte chemoattractant protein-1 and interleukin-8 during the menopausal transition A. Tani1,2, T. Yasui2, K. Kasai3, K. Keyama2, S. Matsui2, T. Kato2, M. Irahara2 1 Department of Obstetrics and Gynecology, The Veritas Hospital, USA 2 Department of Obstetrics and Gynecology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan 3 Department of Obstetrics and Gynecology, Takamatsu Municipal Hospital, Japan 554 women were recruited for the outpatient clinic of the Department of Obstetrics and Gynecology, Tokushima University Hospital. We divided the women into seven stages by menstrual regularity and FSH level: midreproductive stage, late reproductive stage, early menopausal transition, late menopausal transition, very early postmenopause, early postmenopause and late postmenopause. We measured serum concentrations of nine cytokines (IL-1 , IL-5, IL-6, IL-7, IL-8, IL-10, TNF, MIP-1 andMCP-1). IL-8 levels in postmenopausal women were significantly (p=0.001) higher than those in women in the midor reproductive stage and women in early or late menopausal transition. MCP-1 levels in women in late menopausal transition and postmenopause were significantly (p<0.001) higher than those in women in the midor late reproductive stage and women in early menopausal transition. MCP-1 level showed a significant positive correlation (r=0.215, p<0.01) with FSH level in women in menopausal transition. By using a detailed classification of menopausal transition, it was found that the features of IL-8 and MCP-1 levels were differed during the menopausal transition. IL-8 level showed a high level after menopause, while MCP-1 level showed a high level in menopausal transition. MCP-1 may be sensitive to hormonal change andmay be involved in the development of estrogen deficiency diseases. http://dx.doi.org/10.1016/j.jri.2014.09.036 Establishment of new ELISA system for HLA-G N. Ouji-Sageshima1,∗, M. Akasaki2, D.E. Geraghty3, E. Kita4, A. Ishitani5, K. Hatake5, T. Ito1 1 Department of Immunology, Nara Medical University, Japan 2 Akasaki Clinic, Japan 3 Fred Hutchinson Cancer Research Center, USA 4 Department of Microbiology and Infectious Disease Medicine, Nara Medical University, Japan 5 Department of Legal Medicine, Nara Medical University,