Abstract
Objective To study the role of microtubule affinity- regulating kinase 2 (MARK2) in nerve regeneration in an animal model after spinal cord injury (SCI) labeled with biotinylated dextran amine (BDA) using stereotactic injection of. Methods Sixteen SD rats were randomly divided into control group (4 rats) and SCI groups (12 rats). Spinal cord was cut off by scissors in spinal cord injury group. After SCI model was established in 12 rats, normal saline subgroup (4 rats), EGFP-adenovirus subgroup (4 rats), and MARK2-adenovirus subgroup (4 rats) were given the injection of normal saline, EGFP-adenovirus and MARK2-adenovirus into cortical motor area respectively by stereotactic injection technique. One week later, 16 rats were injected with BDA in cortical motor area. After 2 weeks, the animals were executed and the whole brain and spinal cord were removed. Results In SCI groups, rats had lower limb paraplegia (100% success rate). After stereotactic injection of BDA into the motor cortex, the nerve conduction tracts labeled by BDA were confirmed by confocal laser scanning (94% success rate). Conclusion The animal model established by this method is safe, reliable and reproducible. Key words: Spinal cord injury; Model, animal; Nerve regeneration; Biotinylated dextran amine tracing
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