Establishment of mice heterotopic cardiac transplantation and observation between different strains

Abstract

Objective To establish the mice heterotopic cardiac transplantation as well as to observe the different graft reaction between different strains.Methods According to the surgical procedures of Ono's pattern in mice cardiac heterotopic transplantation model,we did allograft cardiac transplantation using same (BALB/c→BALB/c ; CBA→CBA; C57BL/6j→C57BL/6j) or different (BALB/c→C57BL/6j ; C57BL/6j→BALB/c; C57BL/6j→CBA; CBA→C57BL/6j)strains male mice.Survival time and pathological examination were observed after transplantation Results The operative survival was 88.6％ (62/70).Mean donor graft harvest time of was 4.8 min,mean anatomic time was (28.70 ± 3.56) min (BALB/c→BALB/c),(20.70±4.22) min (CBA→CBA) and (27.90±2.51) min (C57BL/6j→C57BL/6j) in same strains while (28.00 ± 2.71) min (BALB/c→C57BL/6j),(22.10 ± 4.33) min (C57BL/6j→CBA),(29.90±3.14) min (C57BL/6j→BALB/c) and (30.33 ±23.00) min (CBA→C57BL/6j) in different strains.The survival time was over 100 days in same strains and (7.10 ±0.74) d (C57BL/6j→BALB/c) ; (7.40 ± 0.82) d (BALB/c→ C57 BL/6j) ; (8.90 ± 1.10) d (C57 BL/6j→ CBA) ; (8.50 ± 0.85) d (CBA→C57BL/6j) d in different strains.It was confirmed with pathological examination.Conclusion On the base of mastering the microsurgical techniques,we can establish the mice cardiac heterotopic transplantation model.The immune response in C57BL/6j to BALB/c model was very strong,while weaker in C57BL/6j to CBA models.The different transplant immune response can be used in the transplantation research. Key words: Mice; Cardiac transplantation; Models ; Strains