Abstract
Objective To establish the metastatic model of human hepatocelular carcinoma (HCC) in nude mice via orthotopic transplantation and further to palliative radiofrequency ablation (RFA) so as to provide a practicable model for the study of biological effects of RFA.Methods Human HCC cell line MHCC97-L was inoculated into 60 BALB/c nude mice.All these cases were divided equally into palliative RFA and sham-operation subgroups.Four weeks after the second operation,the tumor volumes,necrosis range and immunohistochemical staining of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-2 were measured in 7 randomly selected nude mice of each subgroup.Results All nude mice with xenograft tumors exhibited 100.0％ (60/60) transplantability,and 96.7％ (29/30) palliative RFA successful rate was achieved.The expression levels of VEGF and MMP-2 were significantly lower in palliative RFA group than in sham operation group (P ＜ 0.01).Conclusion Metastatic human hepatocellular carcinoma orthotopic transplantation model in combination with palliative RFA in nude mice has a high success rate.After orthotopie tumor transplantation,palliative RFA could reduce the expression of VEGF and MMP-2. Key words: Carcinoma, hepatocellular; Orthotopic implantation ; Radiofrequency ablation ; Model, animal
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
