Abstract
In order to early screen and detect suspected biomarkers from pathogens and the human body itself, tracers or reaction strategies that can act as signal enhancers have been proposed forth at purpose. In this paper, we discussed the applicability of magnetic microparticles-assisted time-resolved fluoroimmunoassay (MMPs-TRFIA) for sensitive determination of potential analytes. Hepatitis B e antigen, antibody to hepatitis B surface antigen and free triiodothyronine were used as biomarker models to explore the reliability of the method. By coupling with bioprobes, MMPs were used as immunoassay carriers to capture target molecules. Under optimal condition, assay performance, including accuracy, precision and specificity, was outstanding and demonstrated satisfactory. To further evaluate the performance of the MMPs-TRFIA in patients, a total of 728 serum samples from hospital were analyzed for three biomarkers in parallel with the proposed method and chemiluminescence immunoassay kit commercially available. Fairly good agreements are obtained between the two methods via data analysis. Not only that but the reliability of MMPs-TRFIA has also been illustrated by three different reaction models. It is confirmed that the novel method modified with MMPs has been established and showed great potential applications in both biological detection and clinical diagnosis, including big molecule protein and low molecular weight haptens.
Highlights
Measurement of different types of serum biomarkers is the foundation of the disease diagnosis and body health
By the determination of purified protein-coated magnetic microparticles (MMPs), about 7.8 μg protein was coupled to per mg MMPs, namely the labeling yield was about 78% and the immunological reaction activity was kept at high level
With the optimization of conditions, the results indicated that the fluorescence intensity increased with incubation time but did not reach a dynamic balance until 30 min and were depicted in S1 Fig. within the time range considered, 30 min was selected as the optimum reaction time for analysis of three biomarkers in subsequent work
Summary
Measurement of different types of serum biomarkers is the foundation of the disease diagnosis and body health. The analysis of biomarkers in human serum offers invaluable information and is essential for the diagnosis of tumor, endocrine, genetic and infectious diseases. Different strategies for rapid and sensitive diagnosis have been discussed and studied increasingly [1,2,3,4]. An optimal platform for serology diagnostics enables detection of a broad array of chemical and biomolecules.
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