Establishment of low temperature perfusion model of animal kidneys in vivo

Abstract

Objective To establish the low temperature perfusion model of pig kidneys in vivo and investigate the protective effect of low temperature perfusion on pig kidney cells. Methods In vivo pig kidneys were randomly divided into 3 groups ( n = 8 ): sham-operation group, low temperature perfusion group and arterial occlusion group. The samples were examined histopathologically. The expression of Caspase-3protein in renal cells was detected by using immunohistochemistry. The ultrastructure of renal tissue was observed under the electron microscopy. Results At the 30th h after occlusion, there was swelling of nuclei and mitochondria and hydropic degeneration of cytoplasma in arterial occlusion group. As compared with sham-operation group and low temperature perfusion group, apoptosis index (AI) [( 1.3 ± 0. 4) %]and the expression of Caspase-3 [( IOD: 2. 42 ± 0. 62)] were significantly increased as compared with arterial occlusion group (P ＜ 0. 05). At the 1st h after occlusion, apoptosis and necrosis of some cells were seen under the ligh microscopy, pyknosis of nuclei, mild lysis of nuclear membrane and severe swelling of mitochondria were observed under the electron microscopy in arterial occlusion group. In low temperature perfusion group, there was no necrosis, and swellsing and vacuolar degeneration of nuclei and mitochondria were observed under the light microscopy. Conclusion This model is easy and convenient, and the low temperature perfusion could protect function of the kidney efficiently. Key words: Low temperature perfusion; Kidney; Model, animal