Establishment of leukemic T-cell lines from mice inoculated with the MAIDS defective virus

Abstract

Mice inoculated with replication-competent stocks of the murine acquired immunodeficiency syndrome (MAIDS) virusare severely immunocompromised and proned to the development of T- and B-cell lymphomas. We have studied the development of T-cell lymphomas in C57BL/6 and RF/J mice inoculated with helper-free stocks of the MAIDS defective virus. We observed the expansion of T cell clones (detected by TCR gene rearrangements and by transplantation) only rarely in diseased C57BL/6 mice and slightly more frequently in RF/J mice. We succeeded in establishing four transplantable T cell tumors and malignant cell lines. The three cell lines from RF/J mice were immature T-cells (Thy-1 +, CD3 −, CD4 +, CD8 +, Mac-1 +), while the line from the C57BL/6 mouse had the phenotype of mature T-cells (Thy-1+, CD3*, CD4 +, CD8 −). All lines were virus-producers despite the fact that helper-free stocks of the virus were inoculated. These helper MuLVs most likely originated from endogenous MuLV sequences. Also, the defective viral genome was clearly detectable in one cell line and was rearranged in two other lines. These established cell lines may be useful to determine whether they share some of the characteristics of the anergic T-cells in vivo and to study the role of the MAIDS defective virus in T cell transformation.