Abstract
ABSTRACTPlants and microbes compete for survival and their organized interactions play a vital role in adapting to stress environments. Microbes have a positive impact on transplanting performance of in vitro propagated plantlets. The present study aims at survival development of micropropagated Pogostemon mollis through microbial inoculation. The leaf and stem explants on MS medium with different concentrations and combinations of BAP, Kn, NAA, IAA and IBA used for the establishment of plantlet. The survival rate of plantlets was successfully increased from 71% to about 70.21% by the inoculation of Rhizophagus fasciculatus and Bacillus megaterium. This inoculation also clearly increases the growth, biomass and chlorophyll of in vitro derived platelets. The present protocol emphasizes the need of bio-hardening on micropropagated plants, particularly the mycorrhization along with phosphobacteria.
Highlights
The Lamiaceae includes aromatic plants that are being used in traditional medicine for various disorders
The present study aims at survival development of micropropagated Pogostemon mollis through microbial inoculation
The survival rate of plantlets was successfully increased from 71% to about 70.21% by the inoculation of Rhizophagus fasciculatus and Bacillus megaterium
Summary
The Lamiaceae includes aromatic plants that are being used in traditional medicine for various disorders. Micropropagation is an established technique for the production of elite plants, owing to transient transplantation shock, plants require biological hardening before transplantation. For this reason, mycorrhizal technology can be applied to reduce transplantation shock during acclimatization, increasing plant survival and establishment rates [7,9]. Mycorrhizae influence survival and growth of micropropagated medicinal plants [10]. This problem can be obviated by combining the technique of micropropagation with mycorrhization during hardening for improved plant survival and growth [11].
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