Establishment of HPLC-RID method for the determination of soluble sugars in peanut seed

Abstract

<p indent=0mm>Edible peanut is one of the important directions of peanut breeding in China and even in the world. However, the lack of efficient detection method of soluble sugar content related to edible quality in peanut kernel limits the progress of edible peanut breeding. In this study, a method of extracting soluble sugar from peanut kernel by 80% ethanol and water bath was established. The method simplified the pre-treatment steps of samples and speeded up the extraction progress compared with the GB method. The accuracy and repeatability tests showed that this method had high repeatability accuracy and effectiveness. Then, the samples extracted by this method and GB method were both tested by HPLC-RID, it was found that the soluble sugar in mature peanut kernel was mainly sucrose, with little glucose and fructose in 20 peanut varieties. There were no significant differences between these two methods. The results of 20 peanut varieties using the method and GB method showed that the sucrose content ranged from <sc>16.19 mg g^-1</sc> to <sc>83.81 mg g^-1,</sc> <sc>15.60 mg g^-1</sc> to <sc>81.38 mg g^-1,</sc> and with an average of <sc>30.41 mg g^-1,</sc> <sc>30.20 mg g^-1.</sc> In summary, the results not only further verified the practicability of the method established in this study, but also showed that there was a huge difference in the sucrose content between these peanut varieties.