Abstract
The Populusxeurumeracunu cv.‘Nan1in895’，an elite clone of Populus deltoades hybrid was selected as areceptor for genetic transformation by use of Cre/IoxP site-specific recombination system (pX6-GFP vector) in this study. The selective markers in the transgenic plants were deleted with different concentration of (3-estradiol induction treatment and by use of two methods respectively in one process from differentiation of leaf discs infected by Agrobucteraum to whole plants and the other process from callus induced by transgenic plants already obtained to plant regeneration. The results showed that the efficiency of selective marker deletion in the process from callus induced by transgenic plants already obtained to plant regeneration was higher than that in the process from ctifferentiation of leaf discs to whole plants. Results of kanamycin sensitivity test showed that there was the same kanamycin sensitivity between selective marker deleted transgenic plants and the control of mlusxeurumeracunu cv.‘Nan1in895'.The system of selective marker-free transformation for poplars was initially established in this study.
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