Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A.

Cao Haiyan,Ma Yuhui,Ge Shaoqin,Liu Yu,Zhang Guowei,Qin Xiude,Zhou Jingna,Liu Chen,Nie Hailiang,Zhao Xinzhe

doi:10.1155/2020/3027843

Abstract

As the main active ingredient in Chinese medicine safflower, hydroxysafflor yellow A (HSYA) has multiple pharmacological effects. In the work, the absorption and fluorescence spectra of HSYA under different environmental conditions (such as acidity, temperature, ions, viscosity, and surfactant) were investigated. The fluorescence intensity of HSYA varied greatly with acidity, temperature, viscosity, and surfactant, but was less affected by common cations and anions. Among various surfactants, we found that borax can significantly enhance the HSYA fluorescence intensity, and thus, a borax-HSYA sensitization system for HSYA fluorescence was established. In the optimized sensitization system, the fluorescence intensity of HSYA increased by 20 times and showed a good linearity with HSYA concentrations in the range of 0∼10 μM with a detection limit of 8 nM. The borax-HSYA sensitization system is nontoxic to T24 cells and mice and can be used for the fluorescence imaging of HSYA in cells, thereby providing an effective method for analyzing HSYA in vitro and monitoring its metabolism in cells.

Highlights

Hydroxysafflor yellow A (HSYA), a chalcone glycoside compound, is the main active ingredient in traditional Chinese medicine of safflower
The fluorescence intensity and stability of HSYA under the combined influence of temperature and light were measured, and the conditions affecting the stability of HSYA were speculated, which provided a reference for the establishment of a highly sensitive fluorescence sensitization analysis method for HSYA
In the fluorescence spectra (Figure 1(b)), HSYA displayed an emission in the range of 490∼650 nm with a peak around 555 nm. e fluorescence intensity remained almost stable in acidic environment, gradually enhanced in the pH range of 7.0∼10.5, and stepwise decreased in the pH range of 10.5∼12.0 (Figure 1(c)). e HSYA solution emitted orange fluorescence under ultraviolet (365 nm) irradiation and reached a maximum level at pH 10.5 (Figure 1(d))

Summary

Introduction

Hydroxysafflor yellow A (HSYA), a chalcone glycoside compound, is the main active ingredient in traditional Chinese medicine of safflower. HSYA has multiple pharmacological effects such as analgesic, anti-inflammatory, antifatigue, antihypoxia, and hypotension [1]. It has broad prospects in the clinical treatment of cardiovascular and cerebrovascular diseases. Erefore, the establishment of analysis method for HSYA is of great significance for studying its pharmacology. High-performance liquid chromatography (HPLC) is the main method for analyzing HSYA. Compared with HPLC, fluorescence analysis has many advantages such as low cost, convenient operation, fast speed, and high sensitivity. In situ imaging and detection of HSYA in biological systems can be achieved with the help of fluorescence microscopy. It is necessary to construct the sensitization system of HSYA to achieve its fluorescence analysis

Methods

Results

Conclusion