Abstract
To establish a model of ovarian cancer with highly lymphatic metastasis in immunocompetent rats. Thirty-two female Fischer 344 rats were divided randomly and equally into two groups: footpad group and intraperitoneal (i.p.) group. At 8 weeks after injection with NuTu-19 ovarian cancer cells, lymphatic metastasis were analyzed by pathohistology; body weight was monitored per week, Survival curves were determined by Kaplan-Meier analysis. Footpad injection could efficiently generate the lymphatic metastasis; specifically, the incidences of metastasis in the ipsilateral popliteal, inguinal and para-iliac lymph nodes were 100% (8/8), 75% (6/8), and 37.5% (3/8), respectively. The mean volume and weight of the ipsilateral popliteal lymph nodes were 0.405 ± 0.096 cm(3) and 0.418 ± 0.118 g in footpad group. However, no lymphatic metastasis lesions were found in i.p. group. Moreover, Kaplan-Meier analysis showed that the average survival time of the footpad group was significantly longer than that of the i.p. group (18.429 ± 1.112 vs. 10.286 ± 0.505 weeks). Our experiments suggest that footpad injection is a very efficient method to generate ovarian cancer with lymphatic metastasis in an immune-competent animal, and we believe that this model will be very helpful for shedding light on the mechanism of lymphogenous metastasis and developing novel therapeutic targets for ovarian cancer patients.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
