Abstract
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate much faster than conventional CHO cells and general mammalian cells. Transgenes could be introduced into CHL-YN cells with high efficiency. Finally, between 50% to > 100% of the amount of glycosylated immunoglobulin G (IgG)1 produced by CHO-K1 cells was produced by CHL-YN cells over a shorter period of time. In summary, fast-growing CHL-YN cells are a unique cell line for producing recombinant proteins.
Highlights
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins
We report the establishment of rapidly growing CHL-YN cells from Chinese hamster lung tissue as a candidate new host cell for producing recombinant proteins
The N-linked glycosylation of biopharmaceutical human immunoglobulin G (IgG) affects antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) activities, which are the main mechanisms of action of antibody drugs[18,19,20]
Summary
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins They are valuable for their ability to grow in serum-free suspension cultures. We established a new cell line from primary cultures of Chinese hamster lung tissue to develop a highly productive host cell that can be a faster growing alternative to conventional CHO cells. To the best of our knowledge, this is the first time a Chinese hamster lung cell line has been established with a clear history of cell culture from seeding tissue to becoming adapted to grow in chemically defined medium; these cells could become important host cells for the biopharmaceutical industry. The colour of the square to the right of the number indicates the combination of fluorochromes of the probes used to recognise each chromosome
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