Abstract
Taiwania ( Taiwania cryptomerioides Hayata), native to Taiwan, is one of the “living fossils” from the Tertiary period of the Cenozoic era. To isolate genes involved in wood formation and biochemical synthesis from this tree, 436 randomly selected clones from a cDNA library derived from seedling were sequenced and analyzed firstly. Contig analysis of these expressed sequence tags (ESTs) identified a total of 246 unigene sets. Based on the results obtained by BLASTX analysis, 10.1% of these ESTs were involved in protein synthesis and processing, including highly expressed small heat shock proteins (smHSPs); 3.9% were related to the cell rescue and defense, including highly expressed cysteine proteinase inhibitors; 0.7% were involved in secondary metabolism. However, 39.0% of ESTs showed no significant similarity to any other protein sequences in public databases. These sequences indicate the uniqueness of Taiwania, and it is revealed that the remarkable value of Taiwania.
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