Abstract
Background Commercial micropropagation of teak (Tectona grandisL.f.) has been achieved for a number of years in countries such as Thailand, India and Malaysia. This has led to the availability of elite genotypes for large scale plantation production. Teak has been shown to develop arbuscular mycorrhizal (AM) fungal associations [1], and this has been attributed to increasing productivity [2]. It is likely that the establishment of mycorrhiza, through the introduction of Glomusspecies, on the roots of micropropagated plantlets will improve the productivity of selected clones. Established hyphal networks can speed up plant colonization [3] and in the long term may also increase carbon storage through glomalin production [4].Our research is pursuing the development of a mycorrhization protocol for micropropagated teak at the acclimatization stage.
Highlights
Commercial micropropagation of teak (Tectona grandisL.f.) has been achieved for a number of years in countries such as Thailand, India and Malaysia
Individual shoots were subsequently exposed to a medium containing 1⁄4 strength M&S macronutrients, full strength M&S micronutrients, 60 mM sucrose, 2.5 gL-1 of agar and 2.5 gL-1 of gelrite [6], and a range of indole butyric acid (IBA) concentrations (0-160 μM) for varying lengths of time (4-28 days) to produce roots in vitro.Rooted plantlets were transferred to three pasteurised soil types (1sand:1perlite;1sand:1peat;and 1sand:1perlite:1peat) under the following conditions: they were maintained under mist
Unprocessed commercial arbuscular mycorrhizal (AM) inoculum 100 g kg-1 from a legume/grass pot culture or processed commercial AM inoculum 10 g kg-1 were both mixed with pasteurised inert soil (1sand:1perlite) and watered to field capacity with 1⁄2 strength sorghum nutrient solution [7]
Summary
Commercial micropropagation of teak (Tectona grandisL.f.) has been achieved for a number of years in countries such as Thailand, India and Malaysia. This has led to the availability of elite genotypes for large scale plantation production. Teak has been shown to develop arbuscular mycorrhizal (AM) fungal associations [1], and this has been attributed to increasing productivity [2]. Established hyphal networks can speed up plant colonization [3] and in the long term may increase carbon storage through glomalin production [4].Our research is pursuing the development of a mycorrhization protocol for micropropagated teak at the acclimatization stage
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